TMET-26. PRIMARY OLIGODENDROGLIOMA CELL CULTURE VIABILITY: AN IN VITRO STUDY WITH METABOLIC MODULATORS

Abstract Oligodendrogliomas are tumors that develop from oligodendrocytes, the myelinating cells of central nervous system. Oligodendrocytes highly metabolically active synthesize and transfer metabolites to neighboring cells. Given its intimate metabolic relation with neurons, we aim investigate oligodendrocyte metabolism as an antitumoral target. A recurred oligodendroglioma WHO grade III was surgically removed a 50-year patient after 10 years progression-free disease. The tumor sample mechanically digested cultured at 37oC, 5% CO2, in DMEM: F12, % FBS antibiotics. Primary were trypsinized seeded 96-well plate. After 24 hs, treated modulators: metformin (MET, mitochondrial complex II inhibitor, 5 mM), 2 deoxyglucose (2DG, hexokinase 1 6-aminonicotinamide (6AN, pentose phosphate pathway 50 µM) and/or 1400W S-methylisothiourea (both iNOS inhibitors, µM, µM respectively). Standard treatment temozolomide (TMZ, 200 also performed. days treatment, stained violet crystal. Two weeks digestion, primary culture successfully established. In vitro, proliferating appeared mostly undifferentiated reduced branching complexity. Hexokinase inhibition by 2DG notoriously affected viability Similar results obtained standard TMZ treatment. On other hand, 6AN did not affect cell monolayer. However, able increase effect MET monotherapy. Both, altered morphology inducing more fusiform shape. Finally, modestly disrupted cell's monolayers this seem be improved combinatory therapies. Glycolytic inhibitor resulted effective against Whereas further studies needed validate these results, better understanding susceptibility could allow development better-targeted more-effective therapeutic approaches.